We have applied data standardization for the analysis of DNA repair capacity using negative and positive internal standards as controls for interexperimental variability. We show that unstimulated cryopreserved PBMCs can be used in DNA repair studies performed using the comet assay. Use of the alkaline comet assay to assess DNA repair capacity in human populations has been limited by several factors, including lack of methodology for use of unstimulated cryopreserved peripheral blood mononuclear cells (PBMCs), insufficient control of interexperimental variability, and limited analysis of DNA repair kinetics. A Modified Alkaline Comet Assay for Measuring DNA Repair Capacity in Human Populations.
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